ABSTRACT
Amfepramone (diethylpropion) is an appetite-suppressant drug used for the treatment of overweight and obesity. It has been suggested that the systemic and central activity of amfepramone produces cardiovascular effects such as transient ischemic attacks and primary pulmonary hypertension. However, it is not known whether amfepramone produces immediate vascular effects when applied in vitro to rat aortic rings and, if so, what mechanisms may be involved. We analyzed the effect of amfepramone on phenylephrine-precontracted rat aortic rings with or without endothelium and the influence of inhibitors or blockers on this effect. Amfepramone produced a concentration-dependent vasorelaxation in phenylephrine-precontracted rat aortic rings that was not affected by the vehicle, atropine, 4-AP, glibenclamide, indomethacin, clotrimazole, or cycloheximide. The vasorelaxant effect of amfepramone was significantly attenuated by NG-nitro-L-arginine methyl ester (L-NAME) and tetraethylammonium (TEA), and was blocked by removal of the vascular endothelium. These results suggest that amfepramone had a direct vasorelaxant effect on phenylephrine-precontracted rat aortic rings, and that inhibition of endothelial nitric oxide synthase and the opening of Ca2+-activated K+ channels were involved in this effect.
Subject(s)
Animals , Male , Acetylcholine/pharmacology , Aorta, Thoracic/drug effects , Appetite Depressants/pharmacology , Diethylpropion/pharmacology , Vasodilator Agents/pharmacology , Aorta, Thoracic/metabolism , Calcium Channels/drug effects , Calcium Channels/metabolism , Endothelium, Vascular/drug effects , NG-Nitroarginine Methyl Ester/metabolism , Nitric Oxide Synthase Type III/drug effects , Phenylephrine/pharmacology , Potassium Channels/drug effects , Potassium Channels/metabolism , Rats, Wistar , Tetraethylammonium/metabolism , Vasoconstrictor Agents/pharmacology , Vasodilation/drug effectsSubject(s)
Infant, Newborn , Humans , Male , Female , Genetic Variation , Genetics, Population , Blood Group AntigensABSTRACT
Se hace un estudio comparativo de cinco sueros antiglobulina humana de amplio espectro o poliespecifico de uso mas frecuente en el pais, procedente de diferentes laboratorios. Se valora su contenido de anti IgG por medio de hematies D positivo y Duffy a (Fya) positivo, sensibilisados con Anti D Anti Fya, respectivamente y su actividad anticomplemento referida principalmente a su reactividad Anti C3d necesaria para el test directo de antiglobulina, cuando se estudian anemias hemoliticas autoinmune